DRUG SENSITIVITY IN 3-D HISTOCULTURED TUMORS

Histocultures are three-dimensional tissue cultures that maintain in-vivo-like phenotype in contrast to monolayer cultures. Histoculture tumor tissue contains cancer cells, stromal elements, immune cells interacting in their native environment. Histocultured tumors can be tested for drug sensitivity to design individualized therapy for each patient or used as a powerful and cost-effective method in drug development [1]. Tumor response to mono or combination treatment could be studied using histoculture drug response assay (HDRA) [2].

Briefly, fresh tumor is sliced into thin pieces and incubated in the in the presence of tested compounds for the established period of time. An efficacy of anti-tumor compound is evaluated on the basis of tumor cell viability and/or any other parameter(s), such as induction of the specific gene expression, connected to the molecular mechanism(s) of compound’s anti-tumor activity. Histoculture could be also a powerful tool for the development of pharmacodynamic markers.

DISASSOCIATED TUMOR CELLS (DTC)

Cureline provides standard DTC specimens from common tumor types (CRC, Breast carcinoma, etc.) and conducts custom projects for fresh tumor processing and DTC preparation from patients with Immuno-Oncology relevant cancers: melanoma, renal carcinoma, pancreatic adenocarcinoma, ovarian cancer, head and neck SCC, etc. These samples contain cancer cells, stromal and other sell types from a malignant tumor and are frozen in DMSO-containing media. These samples are suitable for biomarker studies (FACS analysis, IHC), drug sensitivity evaluation and other functional assays. Cureline Molecular Services provide support to your laboratories in a protocol set up and implementation. 

PBMC FROM PATIENTS WITH SOLID TUMORS

Cureline provides standard PBMC specimens from common tumor types (CRC, Breast carcinoma, NSCLC, etc.) and conducts custom collections of the periferal blood PBMCs from patients with solid tumor types important for Immuno-Oncology projects. We worked in the past with pancreatic carcinoma, renal clear cell carcinoma, ovarian adenocarcinoma, head and neck squamous cell carcinoma,  melanoma and other cancers. Carefully isolated PBMC are frozen in DMSO-containing media and stored in liquid nitrogen. These samples are suitable for biomarker studies and functional assays. Cureline Molecular Services provide support to your laboratories in a protocol set up and implementation. 

References:

1. Hoffman RM. Histocultures and their use. Wiley Online Library. Published Online: 18 Oct 2010
doi: 10.1002/9780470015902.a0002573.pub2
2. Yue G, Wei J, Qian X, Yu L, Zou Z, Guan W, Wang H, Shen J, Liu B. Synergistic anticancer effects of polyphyllin I and evodiamine on freshly-removed tumors.