Primary Human Hepatocytes maintain their morphology and function over a long period of time.
Hepatocytes in suspension are preferable for short-term studies whereas plated hepatocytes are more useful for long-term oriented studies.
Products:
- Fresh (Only Eu) (Plated or in suspension)
- Cryopreserved (Frozen vials)
- 3D Qualified Human Hepatocytes (Frozen Vials)
Common uses:
- Metabolism analysis
- Drug development
- Toxicity assessments
- Enzyme activity studies
Human Non-Parenchymal Cells
Non-parenchymal liver cells (NPCs) makeup 30% of the liver cell population which are composed of Kupffer cells, Stellate cells, and liver Endothelial cells. These NPCs serve to support the structure of the liver, among other functions.
Products:
Kupffer Cells: Kupffer cells are the specialized macrophages residents in the liver, lining the walls of the sinusoids. These cells are part of the mononuclear phagocytes system and can be activated to produce inflammatory cytokines, growth factors and reactive oxygen species.
Hepatic Stellate Cells: Stellate cells are the major cell type involved in liver fibrosis in response to liver injury and they play a key role in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinol metabolism, storage and release.
Liver Endothelial Cells: Liver Endothelial cells (LEC) are highly specialized endothelial cells representing the interface between blood cells and the rest of liver cells. Liver Sinusoidal Endothelial cells (LSEC) are microvascular endothelial cells which, in physiological conditions, regulate hepatic vascular tone contributing to the maintenance of a low portal pressure during digestion.
NPCs Mix: Hepatic non-parenchymal cells (NPCs) represent about 30% of the liver and are specialized cells which interact with hepatocytes for several functions. For this reason, liver co-cultures may increase the sensitivity of liver models for ADME/Tox-related research by recapitulating the complexity of a whole liver. "NPCs Mix" provides a mix of different types of liver cells, including Kupffer cells, liver endothelial cells and Stellate cells.
Common uses:
- Metabolism analysis (time-dependent metabolism)
- Drug interaction
- Liver pathology studies
- Drug metabolism and pharmacokinetics (DMPK)
Culture Media
Cytes media has been designed to successfully culture primary hepatocytes, following specific formulations to support thawing, plating and maintenance of primary cell lines. Most of the products are ready-to-use and are available in a variety of packaging and delivery configurations.
Products:
- Hepatocyte Thawing Media (MHT): Specifically designed for use with thawed cryopreserved hepatocytes to enhance their optimal cell recovery, viability and quality.
- Hepatocyte Plating Media (MHP): Ready-to-use media designed for plating cryopreserved human hepatocytes providing an optimal attachement and monolayer formation.
- Hepatocyte Maintenance Media (MHP): Ready-to-use media designed for the purpose of optimizing the culture of hepatocytes in suspension or plated.
- Hepatocyte Culture Kit (MHTPM): The kit contains media enough for thawing, plating and maintain 1 vial of cryopreserved hepatocytes.
- Hepatocytes 3D - Plating Media (MHP3D): Ready-to-use media designed for plating cryopreserved human hepatocytes providing an optimal 3D formation.
- Hepatocytes 3D - Mantinance Media (MHM3D): Ready-to-use media designed for the purpose of optimize 3D hepatocyte culture.
- Hepatocyte Cryopreservation Media (MHC): Precisely designed to cryopreserve hepatocytes optimizing their viability and quality.
Common uses:
- Optimization of all cell handling and culturing processes to obtain higher yields and superior performance.